Molecular Fluorescence - Principles and Applications

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material,
including two new chapters on applications, which has seen the most signifi cant developments.
 
The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

List of contents

INTRODUCTION
What Is Luminescence?
A Brief History of Fluorescence and Phosphorescence
Photoluminescence of Organic and Inorganic Species: Fluorescence or Phosphorescence?
Various De-Excitation Processes of Excited Molecules
Fluorescent Probes, Indicators, Labels, and Tracers
Ultimate Temporal and Spatial Resolution: Femtoseconds, Femtoliters, Femtomoles, and Single-Molecule Detection
 
PART I: PRINCIPLES
 
ABSORPTION OF ULTRAVIOLET, VISIBLE, AND NEAR-INFRARED RADIATION
Electronic Transitions
Transition Probabilities: The Beer - Lambert Law, Oscillator Strength
Selection Rules
The Franck - Condon Principle
Multiphoton Absorption and Harmonic Generation
 
CHARACTERISTICS OF FLUORESCENCE EMISSION
Radiative and Nonradiative Transitions between Electronic States
Lifetimes and Quantum Yields
Emission and Excitation Spectra
 
STRUCTURAL EFFECTS ON FLUORESCENCE EMISSION
Effects of the Molecular Structure of Organic Molecules on Their Fluorescence
Fluorescence of Conjugated Polymers (CPs)
Luminescence of Carbon Nanostructures: Fullerenes, Nanotubes, and Carbon Dots
Luminescence of Metal Compounds, Metal Complexes, and Metal Clusters
Luminescence of Semiconductor Nanocrystals (Quantum Dots and Quantum Rods)
 
ENVIRONMENTAL EFFECTS ON FLUORESCENCE EMISSION
Homogeneous and Inhomogeneous Band Broadening - Red-Edge Effects
General Considerations on Solvent Effects
Solvent Relaxation Subsequent to Photoinduced Charge Transfer (PCT)
Theory of Solvatochromic Shifts
Effects of Specific Interactions
Empirical Scales of Solvent Polarity
Viscosity Effects
Fluorescence in Gas Phase: Supersonic Jets
 
EFFECTS OF INTERMOLECULAR PHOTOPHYSICAL PROCESSES ON FLUORESCENCE
EMISSION
Introduction
Overview of the Intermolecular De-Excitation Processes of Excited Molecules Leading to Fluorescence Quenching
Photoinduced Electron Transfer
Formation of Excimers and Exciplexes
Photoinduced Proton Transfer
 
FLUORESCENCE POLARIZATION: EMISSION ANISOTROPY
Polarized Light and Photoselection of Absorbing Molecules
Characterization of the Polarization State of Fluorescence (Polarization Ratio and Emission Anisotropy)
Instantaneous and Steady-State Anisotropy
Additivity Law of Anisotropy
Relation between Emission Anisotropy and Angular Distribution of the Emission Transition Moments
Case of Motionless Molecules with Random Orientation
Effect of Rotational Motion
Applications
 
EXCITATION ENERGY TRANSFER
Introduction
Distinction between Radiative and Nonradiative Transfer
Radiative Energy Transfer
Nonradiative Energy Transfer
Determination of Distances at a Supramolecular Level Using FRET
FRET in Ensembles of Donors and Acceptors
FRET between Like Molecules: Excitation Energy Migration in Assemblies of Chromophores
Overview of Qualitative and Quantitative Applications of FRET
 
PART II: TECHNIQUES
 
STEADY-STATE SPECTROFL UOROMETRY
Operating Principles of a Spectrofl uorometer
Correction of Excitation Spectra
Correction of Emission Spectra
Measurement of Fluorescence Quantum Yields
Possible Artifacts in Spectrofl uorometry
Measurement of Steady-State Emission Anisotropy: Polarization Spectra
 
TIME-RESOLVED FLUORESCENCE TECHNIQUES
Basic Equations of Pulse and Phase-Modulation Fluorimetries
Pulse Fluorimetry
Phase-Modulation Fluorimetry
Artifacts in Time-Resolved Fluorimetry
Data Analysis
Lifetime Standards
Time-Resolved Polarization Measurements
Time-Resolved Fluorescence Spectra
Lifetime-Based Decomposition of Spectra
Comparison between Single-Photon Timing Fluorimetry and Phase-Modulation Fluorimetry
 
FLUORESCENCE MICROSCOPY
Wide-Field (Conventional), Confocal, and Two-Pho

About the author

Bernard Valeur received his engineering diploma from the École Supérieure de Physique et de Chimie Industrielles de Paris (E.S.P.C.I.) and his PhD degree from the Université Pierre-et-Marie-Curie (Paris, France), followed by postdoctoral research at the University of Illinois at Urbana-Champaign (USA). After being an associate professor at E.S.P.C.I, he became full professor of physical chemistry at the Conservatoire National des Arts et Métiers (Paris) in 1979, where he is emeritus professor since 2008. Professor Valeur is a member of the laboratory Photophysique et Photochimie Supramoléculaires et Macromoléculaires at the École Normale Supérieure de Cachan since 1996. From 1995 to 2000 he served as an elected member of the French Comité National de la Recherche Scientifique. He is the winner of the 2012 Gregorio Weber Award for Excellence in Fluorescence Theory and Applications and author of over 170 articles or book chapters, five books, and the editor of one book. In addition, he is a member of several editorial boards. Mário Nuno Berberan-Santos graduated in chemical engineering from Instituto Superior Técnico (IST, Technical University of Lisbon, Portugal). After a brief stay at the National Research Council of Canada (Ottawa), he received his PhD in chemistry from IST in 1989. He was a post-doctoral fellow with Bernard Valeur at Conservatoire National des Arts et Métiers (Paris, France), and at Laboratoire pour l'Utilisation du Rayonnement Electromagnétique (Univ. Paris-Sud, Or- say, France). He is full professor of Physical Chemistry at IST, and was invited full professor at the École Normale Supérieure de Cachan (France). He is a member of several editorial advisory boards and is president of the Portuguese Chemical Society (2010-2012). He has authored over 180 publications, including 150 papers in scientific journals, several book chapters, and was the editor of one book.

Summary

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material,
including two new chapters on applications, which has seen the most signifi cant developments.
 
The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Additional text

"The strength of the book lies in its clear and understandable presentation, and in the thoroughness of the descriptions of fluorescence applications, enabling one to quickly appreciate the many questions and problems in the field of fluorescence. Molecular Fluorescence is more a textbook than a monograph, and therefore it is of special interest for students and beginners in the field, and be recommended."
- Angewandte Chemie (international edition), 2002; Vol. 41 No. 16

Report

"The strength of the book lies in its clear and understandable presentation, and in the thoroughness of the descriptions of fluorescence applications, enabling one to quickly appreciate the many questions and problems in the field of fluorescence. Molecular Fluorescence is more a textbook than a monograph, and therefore it is of special interest for students and beginners in the field, and be recommended."
- Angewandte Chemie (international edition), 2002; Vol. 41 No. 16

Product details

• Authors: Mário N. Berberan-Santos, Mario Nuno Berberan-Santos, Mário Nuno Berberan-Santos, Bernar Valeur, Bernard Valeur
• Publisher: Wiley-VCH
• Languages: English
• Product format: Paperback / Softback
• Released: 01.04.2013
• EAN: 9783527328468
• ISBN: 978-3-527-32846-8
• No. of pages: 570
• Dimensions: 170 mm x 30 mm x 240 mm
• Weight: 1067 g
• Illustrations: 4 Farbabb.
• Subjects: Natural sciences, medicine, IT, technology > Chemistry > Physical chemistry; Chemie, Physik, Anorganische Chemie, Spektroskopie, Life Sciences, Biowissenschaften, chemistry, Physics, Photochemistry, spectroscopy, Inorganic Chemistry, Polymer Science & Technology, Polymerwissenschaft u. -technologie, Allg. Polymerwissenschaft u. -technologie, Zell- u. Molekularbiologie, Cell & Molecular Biology, Atom- u. Molekülphysik, Atomic and Molecular Physics, Photochemie, Spezialthemen Physik, Physics Special Topics, Chemie / Anorganische Chemie, Fluoreszenzspektroskopie