Xenotransplantation - Methods and Protocols

Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.

List of contents

Xenotransplantation: An Overview of the Field.-Cloning and Expression Analyses of Pig Genes.-Basic Analyses of Proteins of Interest for Xenotransplantation.-Studies on Carbohydrate Xenoantigens.-Xenoantibodies and Complement Activity Determinations in Pig to Primate Xenotransplantation.-Studies on Coagulation Incompatibilities for Xenotransplantation.-Cellular Studies for in Vitro Modeling of Xenogeneic Immune Responses.-Production of Transgenic and Knock Out Pigs by Somatic Cell Nuclear Transfer.-Small Animal Models of Xenotransplantation.-Heart Xenotransplantation in Primate Models.-Xenogeneic Lung Transplantation Models.-Thymic Transplantation in Pig-to-nonhuman Primates for the Induction of Tolerance Across Xenogeneic Barriers.-Isolation of Porcine Pancreatic Islets for Xenotransplantation.-Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantation.-Hepatocyte Xenotransplantation.-In Vitro Repair Model of Focal Articular Cartilage Defects in Humans.-Potential Zoonotic Infection of PERV in Xenotransplantation.-Ethical and Regulatory Issues for Clinical Trials in Xenotransplantation.-Some Ethical, Social and Legal Considerations of Xenotransplantation.

Summary

Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
 
Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.

Additional text

From the reviews:
“This book gives a comprehensive outline on xenotransplantation, with contributions from a wide range of experts in the field. … The chapters are well-organised and sequenced, and the figures and tables complement the text very well. … it is a well-edited text with a multitude of excellent references. … useful as a reference in a research laboratory, and can be of potential interest to researchers of all grades and levels of expertise in this field.” (Karwan Moutasim, Immunology News, May, 2013)
“This is a methodological book aimed at describing specific topics in xenotransplantation. The purpose is to offer a resource to help those in the field of xenotransplantation or those new researchers interested in starting a career. Given the ongoing need for organs and the advances that could be made with successful xenotransplantation, this is a worthy goal. … this is a thorough book on certain aspects of xenotransplantation, and its methodological approach to certain procedures is helpful to scientists just entering the field.” (Erin C. Maynard, Doody’s Book Reviews, September, 2012)

Report

From the reviews:
"This book gives a comprehensive outline on xenotransplantation, with contributions from a wide range of experts in the field. ... The chapters are well-organised and sequenced, and the figures and tables complement the text very well. ... it is a well-edited text with a multitude of excellent references. ... useful as a reference in a research laboratory, and can be of potential interest to researchers of all grades and levels of expertise in this field." (Karwan Moutasim, Immunology News, May, 2013)
"This is a methodological book aimed at describing specific topics in xenotransplantation. The purpose is to offer a resource to help those in the field of xenotransplantation or those new researchers interested in starting a career. Given the ongoing need for organs and the advances that could be made with successful xenotransplantation, this is a worthy goal. ... this is a thorough book on certain aspects of xenotransplantation, and its methodological approach to certain procedures is helpful to scientists just entering the field." (Erin C. Maynard, Doody's Book Reviews, September, 2012)

Product details

• Assisted by: Cristin Costa (Editor), Cristina Costa (Editor), Cristina Costa Vallès (Editor), Rafael Manez (Editor), Máñez (Editor), Máñez (Editor), Rafael Máñez (Editor), Rafael Manez Mendiluce (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 18.01.2012
• EAN: 9781617798443
• ISBN: 978-1-61779-844-3
• No. of pages: 335
• Weight: 788 g
• Illustrations: XI, 335 p.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subject: Natural sciences, medicine, IT, technology > Biology > General, dictionaries