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Expanded bed adsorption chromatography is a novel processing technique for the purification of biomolecules, combining clarification, concentration and initial purification in one step. By such an integration it is possible to reduce the number of steps in the purification process, to shorten the processing time and to improve the yields.
The technology is new, and interesting developments have taken place concerning the adsorbents, the processing technology and potential applications. Both small-scale laboratory processes and larger industrial processes are being developed.
Expanded bed chromatography is one of the most exciting new developments in downstream processing in recent years. The technology will be a standard procedure when new biotechnological processes are being developed.
List of contents
Expanded bed adsorption: elution in expanded bed mode.- Monitoring, modeling, and control strategies for expanded-bed adsorption processes.- Factors affecting dispersion in expanded bed chromatography.- Physical and biochemical characterization of a simple intermediate between fluidized and expanded bed contactors.- On-line monitoring of the purification of GST-(His)6 from an unclarified Escherichia coli homogenate within an immobilised metal affinity expanded bed.- The effect of column verticality on separation efficiency in expanded bed adsorption.- Evaluation of the effect of in-bed sampling on expanded bed adsorption.- EBA columns with a distribution system based on local stirring.- Simplified and more robust EBA processes by elution in expanded bed mode.- Cell/adsorbent interactions in expanded bed adsorption of proteins.- Validation issues related to expanded bed technology.- Highly dense beaded sorbents suitable for fluidized bed applications.- Characterization of very dense mineral oxide-gel composites for fluidized-bed adsorption of biomolecules.- Expanded bed adsorption on supermacroporous cross-linked cellulose matrix.- Characterisation of STREAMLINETM Phenyl.- Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins.- A simplified purification procedure of a-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography.- Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media.- Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption.- Capture of human Fab fragments by expanded bed adsorption with a mixed mode adsorbent.- Purification of Recombinant Human Serum Albumin. Efficient purification usingSTREAMLINE.- Direct coupling of expanded bed adsorption with a downstream purification step.- Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography.- Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli.- On-line monitoring of glucose and/or lactate in a fermentation process using an expanded micro-bed flow injection analyzer.- Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column.- Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis.- Flow injection analysis of intracellular ?-galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification.- Key word Index.- Author Index.
