Intelligent Surfaces in Biotechnology - Scientific Engineering Concepts, Enabling Technologies, Translation

Informationen zum Autor H. Michelle Grandin currently works as a scientific writer and consultant in the field of tissue regeneration and implant surfaces for dental applications. Previously, she worked as a senior research scientist in the biointerface group of Marcus Textor at ETH Zurich. There, she developed a waveguide fluorescence microscope for the study of bio-interactions in the near-field as well as leading a group in the development of 3D single cellular microwell arrays for application in stem cell and cancer research. Marcus Textor is a Professor Emeritus at ETH Zurich, Department of Materials. He was head of a research group dedicated to surfaces and interfaces in bio-related fields of material science. His research interests cover both fundamental aspects of the behavior of materials in contact with biological milieus and the design and development of surfaces that elicit biospecific responses. Klappentext A comprehensive overview of smart and responsive surfaces in biotechnology and their applicationsA wave of recent advances in cell biology, biophysics, chemistry, and materials science has enabled the development of a new generation of smart biomaterials. Intelligent Surfaces in Biotechnology: Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications provides readers with a comprehensive overview of surface modifications and their applications, including coverage of the physico-chemical properties, characterization methods, smart coating technologies, and demonstration of performance in vitro and in vivo.The first part of the book covers applications in the fields of biosensing and biodiagnostics, while the second part focuses more on coatings for medical devices, drug delivery, and tailored cell-surface interactions. The book explores intelligent surface applications such as tissue engineering, drug targeting and delivery, wound healing and anti-infection strategies, biosensors, nanopatterning, and bioinspired design of novel responsive materials and multifunctional surfaces.Designed to aid scientists and engineers in understanding the rapidly developing field of biofunctional surfaces, Intelligent Surfaces in Biotechnology is an edited volume with each chapter written by a respected expert and featuring examples taken from the most state-of-the-art developments in the discipline.Cover Image: Design concept for a diagnostic microfluidic system based on responsive polymer- and antibody-conjugated nanobeads (see Chapter 2 of this book, Figure 2.5; reproduced by permission from the Royal Society of Chemistry). Zusammenfassung This resource gives a comprehensive overview of surface modifications for applications in biotechnology using intelligent coatings. The coverage includes chemical properties, characterization methods, coating techniques, state-of-the-art examples, and an outlook on the promising future of this technology. Inhaltsverzeichnis Foreword xv Preface xix Contributors xxiii 1. Stimulus-Responsive Polymers as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms, and Applications 1 Vinalia Tjong, Jianming Zhang, Ashutosh Chilkoti, and Stefan Zauscher 1.1 Introduction 1 1.2 SRP Architectures for Biosensor Applications 2 1.2.1 Cross-Linked Polymer Networks (Hydrogels) 2 1.2.2 End-Grafted Polymer Chains (Polymer Brushes) 5 1.2.3 Self-Assembled Polyelectrolyte (PEL) Multilayers (LBL Thin Films) 5 1.2.4 Molecularly Imprinted Polymers 6 1.2.5 Hybrid Coatings 6 1.3 Mechanisms of Response 6 1.3.1 Sensing Selectivity 6 1.3.2 Conformational Reorganization of SRP Coatings 7 1.3.2.1 Changes in Osmotic Swelling Pressure 7 1.3.2.2 Changes in Apparent Cross-Link Density 8 1.4 Sensing and Transduction Mechanisms 9 1.4.1 Optical Tran...

List of contents

Foreword xv
 
Preface xix
 
Contributors xxiii
 
1. Stimulus-Responsive Polymers as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms, and Applications 1
Vinalia Tjong, Jianming Zhang, Ashutosh Chilkoti, and Stefan Zauscher
 
1.1 Introduction 1
 
1.2 SRP Architectures for Biosensor Applications 2
 
1.2.1 Cross-Linked Polymer Networks (Hydrogels) 2
 
1.2.2 End-Grafted Polymer Chains (Polymer Brushes) 5
 
1.2.3 Self-Assembled Polyelectrolyte (PEL) Multilayers (LBL Thin Films) 5
 
1.2.4 Molecularly Imprinted Polymers 6
 
1.2.5 Hybrid Coatings 6
 
1.3 Mechanisms of Response 6
 
1.3.1 Sensing Selectivity 6
 
1.3.2 Conformational Reorganization of SRP Coatings 7
 
1.4 Sensing and Transduction Mechanisms 9
 
1.4.1 Optical Transduction 9
 
1.4.2 Electrochemical Transduction 14
 
1.4.3 Mechanical Transduction 17
 
1.5 Limitations and Challenges 19
 
1.5.1 LOD and Sensitivity 19
 
1.5.2 Selectivity 20
 
1.5.3 Working Range 20
 
1.5.4 Response Time 20
 
1.5.5 Reliability and Long-Term Stability 21
 
1.6 Conclusion and Outlook 22
 
Acknowledgements 22
 
References 22
 
2. Smart Surfaces for Point-of-Care Diagnostics 31
Michael A. Nash, Allison L. Golden, John M. Hoffman, James J. Lai, and Patrick S. Stayton
 
2.1 Introduction 31
 
2.1.1 POC Testing Challenges 32
 
2.2 Standard Methods for Biomarker Purification, Enrichment, and Detection 33
 
2.3 Smart Reagents for Biomarker Purification and Processing 34
 
2.3.1 IgG Antibody-pNIPAAm Conjugates 38
 
2.3.2 Single-Chain Antibody-pNIPAAm Conjugates 39
 
2.3.3 Nucleotide-pNIPAAm Conjugates 40
 
2.3.4 Magnetic Nanoparticle (mNP)-pNIPAAm Conjugates 40
 
2.3.5 Gold Nanoparticle (AuNP)-pNIPAAm Conjugates 42
 
2.4 Sample-Processing Modules for Smart Conjugate Bioassays 44
 
2.4.1 Grafting of pNIPAAm from Microchannel Surfaces 45
 
2.4.2 Grafting of pNIPAAm from Porous Membranes 48
 
2.4.3 Magnetic Processing Modules 51
 
2.5 Devices for Use in Smart Conjugate Bioassays 54
 
2.5.1 Lateral-Flow Immunochromatography Devices 55
 
2.5.2 Wicking Membrane Flow-Through Devices 56
 
2.5.3 Polylaminate Microfl uidic Devices 57
 
2.5.4 Multilayer PDMS Smart Microfl udic Devices 58
 
2.6 Conclusions 60
 
References 61
 
3. Design of Intelligent Surface Modifications and Optimal Liquid Handling for Nanoscale Bioanalytical Sensors 71
Laurent Feuz, Fredrik Höök, and Erik Reimhult
 
3.1 Introduction 71
 
3.2 Orthogonal Small (Nano)-Scale Surface Modification Using Molecular Self-Assembly 75
 
3.2.1 Surface Anchor: How to Define and Retain a Molecular Pattern 77
 
3.2.2 Spacer: How to Suppress Binding 83
 
3.2.3 Recognizing and Capturing Analytes on an Intelligent Nanostructure 86
 
3.3 Alternative Surface Patterning Strategies 89
 
3.3.1 Lithographic Patterning of Physisorbed Macromolecules 89
 
3.3.2 Nanoscale Molecular Surface Modification through Printing 90
 
3.3.3 Nanoscale Molecular Surface Modification through Direct Writing 91
 
3.3.4 Multivalency and the Intelligent Fluid Biointerface 92
 
3.3.5 Summary Functionalization of Nanoscale Biosensors 95
 
3.4 The Challenge of Analyte Transport 95
 
3.4.1 Convective versus Diffusive Flux ( jC vs. jD) 98
 
3.4.2 Reactive versus Diffusive Flux ( jR vs. jD) 106
 
3.4.3 Design and Operation Criteria for Efficient Mass Transport 108
 
3.5 Conclu

Report

"The present book with its 350 pages is not a textbook but reading for experts and inspires scientists and engineers to further investigations to tackle the transition from synthetic to living materials, äs it is meant by the term "intelligent interfaces". The presented "smart" biosensors and medical devices demonstrate nicely, how we can learn from nature and make profit of its impressive "inventions" in our modern life." ( Tenside Surfactants Detergents , 1 May 2012)

Product details

• Authors: H Michelle Grandin, H. Michelle Grandin, H. Michelle Textor Grandin, Marcu Textor, Marcus Textor, Marcus Grandin Textor, TEXTOR MARCUS GRANDIN H MICHELLE
• Assisted by: H. Michelle Grandin (Editor), Grandin H. Michelle (Editor), Marcus Textor (Editor), Textor Marcus (Editor), Whitesides George M. (Foreword)
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Hardback
• Released: 23.03.2012
• EAN: 9780470536506
• ISBN: 978-0-470-53650-6
• No. of pages: 400
• Subjects: Natural sciences, medicine, IT, technology > Medicine > General; Life Sciences, Biowissenschaften, biotechnology, Biomaterial, Materialwissenschaften, Materials science, Polymer Science & Technology, Polymerwissenschaft u. -technologie, Allg. Polymerwissenschaft u. -technologie, Thin Films, Surfaces & Interfaces, Dünne Schichten, Oberflächen u. Grenzflächen, Biotechnologie i. d. Biowissenschaften