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Klappentext This newest edition to the Laboratory Techniques Series gives current state of the art use of synthetic peptides in molecular biology and practical protocols on how to conjugate peptides, immunize animals with peptides and monitor immune responses to peptides in vitro. It gives background information on antigenic specificity, prediction of antigenic sites in proteins and applications of peptides in immunology and virology, as probes in diagnosis and as vaccines. The book also describes antigenicity of proteins and methods to localize antigenic sites as well as methods for predicting epitoxes, and gives detailed protocols for peptide-carrier conjugation, immunization with peptides, and peptide immunoassays. The volume also describes typical use of antipeptide antibodies in molecular and cellular biology as well as the use of peptides in the diagnosis of viral infections and autoimmune diseases, and the use of peptides as potential synthetic vaccines. An excellent edition to an excellent series, available in hardbound and paperback. Inhaltsverzeichnis 1. Molecular Dissection of Protein Antigens and the Prediction of Epitopes 2. Peptide-Carrier Conjugation 3. Immunization with Peptides 4. Peptide Immunoassays 5. Use of Antipeptide Antibodies in Molecular and Cellular Biology 6. The Use of Peptides for Diagnosing Viral Infections 7. Peptides in Diagnosis of Autoimmune Diseases 8. Synthetic Peptides as Vaccines
List of contents
1. Molecular Dissection of Protein Antigens and the Prediction of Epitopes
2. Peptide-Carrier Conjugation
3. Immunization with Peptides
4. Peptide Immunoassays
5. Use of Antipeptide Antibodies in Molecular and Cellular Biology
6. The Use of Peptides for Diagnosing Viral Infections
7. Peptides in Diagnosis of Autoimmune Diseases
8. Synthetic Peptides as Vaccines
Report
"Immunoassay of peptides, their use for different types of diagnosis, and the evaluation of synthetic peptides as vaccines all point to the interest of this book both at a fundamental level as well as in terms of applications. (...)useful illustrations can be found in its over 300 pages." --Res. Microbiology Vol. 151
"This work is strongly recommended to all clinical, diagnostic and research Immunologists and Immunochemists." --Immunological Invest., Vol. 29, No. 4
