Read more
Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states. In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world. As a volume in the highly successful Methods in Molecular Biology(TM) series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.
List of contents
Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations.- Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell.- Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus.- Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity.- Analysis of Cell Shape and Polarity during Zebrafish Gastrulation.- Analyzing Planar Cell Polarity during Zebrafish Gastrulation.- Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation.- Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling.- Gene Loss-of-Function and Live Imaging in Chick Embryos.- Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation.- Convergent Extension Analysis in Mouse Whole Embryo Culture.- Analysis of PCP Defects in Mammalian Eye Lens.- Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea.- Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells.- The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration.- Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells.- Regulation of Focal Adhesion Dynamics by Wnt5a Signaling.- The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity.- Assessing PCP in the Cochlea of Mammalian Ciliopathy Models.- Morphometric Analysis of Centrosome Position in Tissues.
Summary
Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states. In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.
