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Zusatztext The third edition of this classic guide to protein purification updatesmethods! principles and references. As in the widely-acclaimed earliereditions! Scopes guides both the novice and the experienced researcherfrom theory to application. Using the book! the reader will be able tointegrate methods effectively into optimum protocols for the task at hand.Reviews of earlier editions of Protein Purification described it as "goodpractical advice that is presented in a pleasantly readable form" (AnalyticalBiochemistry)! "well organized and written clearly" (American Scientist)!and "should be on every laboratory shelf where proteins are being handledor purified... a feast and a genuine pleasure to read" (Nature). Klappentext The third edition of this classic guide to protein purification updates methods! principles and references. As in the widely-acclaimed earlier editions! Scopes guides both the novice and the experienced researcher from theory to application. Using the book! the reader is able to integrate methods effectively into optimum protocols for the task at hand.Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry"well organized and written clearly" --American Scientist"should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature Zusammenfassung New textbooks at all levels of chemistry appear with great regularity. Our goal! in this series! is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks! and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. Inhaltsverzeichnis Prefaces; 1. The Protein Purification Laboratory; 2. Making an Extract; 3. Analysis- Measurement of Protein and Enzyme Activity; 4. Separation by Precipitation; 5. Separation by Adsorption I: General Principles; 6. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents; 7. Separation by Adsorption: Affinity Techniques; 8. Separation in Solution; 9. Purification of Special Types of Proteins; 10. Small-Scale and Large-Scale Procedures; 11. Analysis for Purity; 12. Optimization of Procedures: Final Steps; Appendix A-D; References; Index ...
List of contents
1 The Protein Purification Laboratory.- 2 Making an Extract.- 3 Analysis-Measurement of Protein and Enzyme Activity.- 4 Separation by Precipitation.- 5 Separation by Adsorption I: General Principles.- 6 Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents.- 7 Separation by Adsorption-Affinity Techniques.- 8 Separation in Solution.- 9 Purification of Special Types of Proteins.- 10 Small-Scale and Large-Scale Procedures.- 11 Analysis for Purity.- 12 Optimization of Procedures; Final Steps.- Appendix A.- Precipitation Tables.- Appendix B.- Solutions for Measuring Protein Concentration.- Appendix C.- Buffers for Use in Protein Chemistry.- Appendix D.- Chromatographic Materials.- References.
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From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)
