Read more
Informationen zum Autor NAWIN C. MISHRA, PhD, is Professor in the Department of Biological Sciences at the University of South Carolina. He was elected a Fellow of the American Association for the Advancement of Science in 1986 for his original contribution to the study of gene transfer in fungi. Klappentext An authoritative study of a cornerstone of cutting-edge DNA technology Nucleases are a class of enzymes that catalyze the hydrolysis of nucleic acids (DNA, RNA) in all organisms, including humans. In addition to their important biological role, nucleases have recently emerged as useful tools in laboratory studies, and have led to the development of such fields as recombinant DNA technology, molecular cloning, and genomics. Nawin Mishra?s Nucleases, the first comprehensive treatment of the subject, introduces the properties and biological roles of nucleases to newcomers in the field and provides the basis of their possible application to critical aspects of science, commerce, and industry. Covering the structure and biochemical properties of nucleases, Mishra?s text further guides the reader to understand how these enzymes can be exploited to make new products for the diagnosis and treatment of disease. Molecular cloning, made possible by the evolving understanding and application of nucleases, may well lead to the identification and characterization of genes responsible for diseases and their possible alleviation by gene therapy and the development of designer drugs. Nucleases is a one-stop, authoritative resource for students of biology and researchers currently practicing in the field. Chapter topics include: Ribonucleases Restriction Endonucleases Topoisomerases Sugar Non-Specific Nucleases Molecules That Interact with Nucleases An ideal text and reference work for professionals and students in biochemistry, genetics, and molecular biology, Nucleases promises to be the field?s standard-bearer for years to come. Zusammenfassung Provides detailed applications of nucleases in recombinant DNA technology, molecular cloning, biotechnology, pharmaceuticals, and commerce. This work covers the role of nucleases in biological systems, with focus on understanding their role in causing human diseases. Inhaltsverzeichnis Preface. List of Nobel Prize Winners for Their Research Work with Nucleases. About the Author. 1. Introduction. I. Historical Perspectives. II. Protein, RNA, DNA, and Other Molecules as Nucleases. III. Nature of Enzymatic Reactions Catalyzed by Nucleases. IV. Classification. A. Nature of Substrates. B. Mode of Attack. C. Site-Specificity and Structure-Selectivity. V. Methods for the Study of Nucleases. A. Methods for the Assay of the Enzymatic Activity. B. Methods for the Study and Characterization of Nucleases. VI. Genetics of Nucleases and Biological Roles. VII. Applications of Nucleases. 2. Ribonuclease. I. General Ribonucleases. A. Microbial Ribonucleases. 1. RNaseT1. 2. RNaseT2. B. Mammalian Ribonucleases. 1. Bovine Pancreatic Rnase. 2. RNaseA. 3. Human Pancreatic Ribonuclease (HPR). 4. Human Nonsecretory Ribonuclease (HNSR). 5. Human Major Basic Protein (MBP), Eosinophil Cationic Protein (ECP) and Eosinophil-Derived Neurotoxin (EDN). 6. Angiogenin. 7. Interferon-Induced Mammalian Ribonuclease. 8. Human RNase with a Possible Role in Tumor Suppression. C. Plant Ribonucleases. D. Evolution of Ribonucleases. II. Ribonucleases Involved in RNA Processing (Trimming, Splicing, and Editing). A. RNaseIII and RNaseIII-Like Enzymes. B. RNaseP. C. RNaseE. D. RNaseM5. E. RNaseD. F. Eukaryotic RNA-Splicing Enzymes. 1. Yeast tRNA Splicing Endonuclease. III. Ribonuclease H. A. E. coli RN...
