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Inhaltsverzeichnis Table of Contents DIAGNOSTIC PRINCIPLES I. Molecular Hybridization for Microbial Identification 1. Applications of Fluorescence In Situ Hybridization in Diagnostic Microbiology Stefan Juretschko and Thomas R. Fritsche 2. DNA Probes for Culture Confirmation and Direct Detection of Bacterial and Fungal Infections: a Review of Current Technologies and Assays Julie Kingery and Karen C. Carroll II. Nucleid Acid Amplification Methods 3. In Vitro Nucleic Acid Amplification Techniques Vivekanand Datta and Randall T. Hayden 4. Real Time PCR and Melting Analysis Carl T. Wittwer and Noriko Kusukawa 5. Quantitative Molecular Methods Donna M. Wolk and Randall T. Hayden 6. Use of Commercial Amplification Tests in the Clinical Microbiology Laboratory: Test Selection and Quality Assurance Gerri S. Hall, Mark Katanik, Marion Tuohy, and Mary Sholtis III. Laboratory Operations 7. Nucleic Acid Isolation: Overview of Sample Preparation Methods Charles E. Hill 8. Laboratory Design and Operations P. Shawn Mitchell, Jeffrey J. Germer, and Joseph D. C. Yao IV. Molecular Typing Methods 9. Molecular Phylogenetic Analysis Les Dethlefsen, Paul W. Lepp, and David A. Relman 10. Pulsed-Field Gel Electrophoresis: Laboratory and Epidemiologic Considerations for Interpretation of Data Richard V. Goering, Efrain M. Ribot, and Peter Gerner-Smidt 11. Strain Typing Using Multiple Loci "Variable Number of Tandem Repeat" Analysis and Genetic Element CRISPR Christine Pourcel and Gilles Vergnaud 12. Repetitive Sequence-Based PCR Typing of Bacteria and Fungi Stacie R. Frye and Mimi Healy 13. Molecular Typing Methods for Analysis of Extraintestinal Pathogenic Escherichia coli James R. Johnson V. Detection Methodology 14. Fluorescence Resonance Energy Transfer J. R. Uhl, Y.-W. Tang, and F. R. Cockerill III 15. Detection of Pathogenic Organisms with Multicolor Molecular Beacons Fann Wu, Phyllis Della-Latta, Sanjay Tyagi, and Fred Russell Kramer 16. Microwell Plate Detection Systems for Amplicon Detection and Characterization Jiang Fan and Michael J. Loeffelholz 17. Detection Methodology: Pyrosequencing Liying Yan and Robert Slinger 18. Solid- and Liquid-Phase Array Technologies Melissa B. Miller VI. New Technologies 19. Next Generation DNA Sequencing and Microbiology Russell Higuchi, Ulf Gyllensten and David H. Persing 20. Raman Spectroscopy for Bacterial Strain Typing Diana Willemse-Erix, Alex van Belkum, and Kees Maquelin 21. Multiplex PCR Product Detection and Discrimination Steven Zink, Nick M. Cirino, and Christina Egan 22. Detection of Emerging Antimicrobial Resistance by Use of the Ibis T5000 Universal Biosensor David J. Ecker, Rangarajan Sampath, Christian Massire, Lawrence B. Blyn, Mark W. Eshoo, Thomas A. Hall, and Steven Hofstadler DIAGNOSTIC APPLICATIONS VII. Molecular Detection of Bacterial Organisms 23. Update on the Detection and Characterization of Bacterial Pathogens by Nucleic Acid Amplification K. Loens, H. Goossens, and M. Ieven 24. Molecular Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Jens K. Moller, Bjorn Herrmann, Jorgen Skov Jensen, and Henrik Westh 25. PCR Detection of Haemophilus ducreyi, Treponema pallidum and Mycoplasma genitalium Patricia A. Totten, Lisa E. Manhart, and Arturo Centurion-Lara 26. Molecular Detection and Characterization of Mycobacterium tuberculosis Betty A. Forbes 27. Sequence-Based Identification and Characterization of Mycobacteria Nancy L. Wengenack and Leslie Hall 28. Molecular Detection of Group B Streptococcus Elizabeth M. Marlowe and Preeti Pancholi 29. Molecular Detection and Identification of Methicillin-Resistant Staphylococcus aureus Willem van Leeuwen and Alex van Belkum 30. Identification of Bacteria by DNA Target Se...
