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Mineralizing Vesicles: From Biochemical and Biophysical Properties to Their Roles in Physiology and Disease presents the state-of-the art in the properties of mineralizing EVs and their potential clinical applications.
The first chapter presents foundational biochemical and biological aspects of EVs. Next, the title coves the role of EVs in bone ossification and in cardiovascular and cartilage-related diseases. Considering the unique ability of this class of EVs to form apatite minerals assigned to their special biochemical machinery, three chapters of the book then focus on the enzymes catalysing the inorganic phosphate and calcium turn-over and the dynamic properties of the vesicles' peripheral proteins. Chapters describe the role of inorganic phosphate and calcium ions and of autophagy on the biogenesis and function of mineralizing EVs. Recent studies show that the lumen of mineralizing EVs is partially filled with miRNA, and a chapter therefore considers research on the possible function of these vesicles as signalosomes. The final five chapters of the book describe practical aspects of working with mineralizing EVs, including their purification, proteomic and biophysical analyses, the use of biomimetic models and mineralizing EVs in regenerative medicine.
This title presents, for the first time, a comprehensive account of mineralizing EVs and their potential clinical applications. It will be invaluable to researchers in the field.
Über den Autor / die Autorin
Massimo Bottini is Associate Professor of Biochemistry at the University of Rome Tor Vergata (Rome, Italy) since 2015. As previous positions, he has been Affiliate Associate Professor at the Sanford Burnham Prebys (La Jolla, USA) from 2015 to 2022, and Visiting Professor at the University of the Chinese Academy of Sciences (Beijing, People’s Republic of China) from 2017 to 2019 and at the University of São Paulo (São Paulo, Brazil) in 2020. He has been the recipient of fellowships/grants from the Juvenile Diabetes Research Foundation, the Arthritis National Research Foundation, the European Commission, the University of Rome Tor Vergata, and the Chinese Academy of Sciences. He leads the Laboratory of Biochemical Nanotechnology at the University of Rome Tor Vergata. His research is mostly focused on the characterization of the biochemical and biophysical properties of the extracellular vesicles released during physiologic and ectopic biomineralization processes.Ana Paula Ramos is an Associate Professor at the University of Paulo-Brazil. She got her first degree in Chemistry in 2004 and a Ph.D. in Physical Chemistry in 2009. She is currently an associate Professor of Physical Chemistry, at the University of São Paulo Brazil and a researcher of the Brazilian National Council for Scientific and Technological Development (CNPq). She is the coordinator of the Physical Chemistry of Colloids and Surfaces Laboratory, with expertise in surfaces modification and characterization by microscopic and spectroscopic techniques. Her main research interests are the synthesis of biomaterials for bone replacement and regeneration. In special, her current projects focus on the use of biomimetic matrices inspired by the interaction of phospholipids and osteogenic proteins found in matrix vesicles as modifiers of metals used for dental implants. Moreover, she is currently investigating the role of strontium on physiological and pathological mineralization.
