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This book explores in depth the purification and analysis of the Vitamin B12 / Haptocorrin complex, using state-of-the-art techniques such as X-ray absorption spectroscopy (XAS). After an introduction to vitamin B12 and haptocorrin, it details the steps involved in purifying the complex, from isolating haptocorrin to forming the complex with vitamin B12, via purification and concentration. The book also describes sample preparation methods for XAS, and data collection and analysis processes. The use of XAS in the study of complex structure is discussed, while current technological challenges and future prospects for the study of these protein complexes in biomedical applications are addressed. This book aims to provide a comprehensive resource for researchers in biochemistry, biophysics and spectroscopy.
About the author
Dr. Mimouni ist ein unabhängiger Forscher mit Schwerpunkt auf bioinorganischer Chemie und XAS-Experte. Er promovierte 1997 in Chemie an der Universität Paris XII, nachdem er 1993/92/91 ein Diplôme d'Études Approfondies en Systèmes Bioinorganiques, einen Maîtrise und einen Licence in Chemie an der Universität Paris XI absolviert hatte.
