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With genetic engineering, systems explored in this book now exist allowingfor the simple, efficient, and near universally precise genetic manipulationdirectly in any organism, including the mouse. Herein, these models are appliedto a wide field of disease areas, including diabetes, cardiovascular disease,skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinaldisorders, as well as various behavioral models. Written for the highlysuccessful Methods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Practicaland fully updated, Mouse Models for DrugDiscovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overviewof techniques to utilize the many possibilities of mice in the drug developmentprocess.
List of contents
Genetically Defined Strains in DrugDevelopment and Toxicity Testing.- Simple, Efficient CRISPR-Cas9Mediated Gene Editing in Mice: Strategies and Methods.- Mouse Sperm Cryopreservation andRecovery of Genetically Modified Mice.- Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftmentof Hematopoietic Stem Cells.- Generation of Human Liver ChimericMice for the Study of Human Hepatotropic Pathogens.- Human FcRn Transgenic Mice forPharmacokinetic Evaluation of Therapeutic Antibodies.- A Humanized Mouse Model to StudyHuman Albumin and Albumin Conjugates Pharmacokinetics.- Germ-Free Mice Model for StudyingHost-Microbial Interactions.- Bridging Mice to Men: Using HLATransgenic Mice to Enhance the Future Prediction and Prevention of AutoimmuneType 1 Diabetes in Humans.- Mouse Models of Type 2 DiabetesMellitus in Drug Discovery.- Cholesterol Absorption andMetabolism.- Skin Diseases in Laboratory Mice:Approaches to Drug Target Identification and Efficacy Screening.- Chronic Myeloid Leukemia (CML) MouseModel in Translational Research.- Murine Model for Colitis-AssociatedCancer of the Colon.- Mouse Models for StudyingDepression-Like States and Antidepressant Drugs.- Experimental Models of Anxiety forDrug Discovery and Brain Research.- Repetitive Behavioral Assessmentsfor Compound Screening in Mouse Models of Autism Spectrum Disorders.- Better Utilization of Mouse Modelsof Neurodegenerative Diseases in Preclinical Studies: From the Bench to theClinic.- Neuromuscular Disease Models andAnalysis.- Brightfield Imaging and Optical Coherence Tomography of the MousePosterior Eye.- MouseModels as Tools to Identify Genetic Pathways for Retinal Degeneration, asExemplified by Leber's Congenital Amaurosis.
Summary
With genetic engineering, systems explored in this book now exist allowing
for the simple, efficient, and near universally precise genetic manipulation
directly in any organism, including the mouse. Herein, these models are applied
to a wide field of disease areas, including diabetes, cardiovascular disease,
skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal
disorders, as well as various behavioral models. Written for the highly
successful Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical
and fully updated, Mouse Models for Drug
Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview
of techniques to utilize the many possibilities of mice in the drug development
process.
