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Informationen zum Autor Bryan M. Ham, PhD, is a member of the American Society for Mass Spectrometry, the American Chemical Society, the Tear Film and Ocular Surface Society, and the Association for Research in Vision and Ophthalmology. He is currently conducting proteomics research at Pacific Northwest National Laboratory in Richland WA. His research interests include biomolecule analysis in the areas of proteomics, lipidomics, and metabolomics. Klappentext The analysis of even electron mass spectra for unknown biomolecule structure identification and elucidationIn addition to providing a practical introduction to mass spectrometry, this bookaddresses the spectral interpretation of even electron mass spectra generated from soft ionization techniques-a fast-growing area with tremendous potentialin biomolecule analysis. From the basics and principles of mass spectrometry to specific applications with examples, Even Electron Mass Spectrometry with Biomolecule Applications covers:*The fundamentals of mass spectrometry, including the approved mass analyzers, reaction rate, and collision theory*Diverse ionization techniques and sources, including electron ionization and the more current soft ionization techniques of electrospray ionization and matrix assisted laser desorption ionization*The basic skills and techniques needed for mass spectral interpretation of even electron ions*Both precursor and product ion mass spectra*Applications to biomolecule analyses, including the analysis of proteins, peptides, DNA/RNA, metabolites, lipids, and carbohydratesIn addition to the essential theoretical background and fundamental principles, this unique reference presents a detailed, step-by-step methodology for interpreting even electron mass spectrometry results. Specific chapters are devoted to: proteomics; biomolecule spectral interpretation of small molecules; biomolecule spectral interpretation of biological macromolecules; and MALDI-TOF-Postsource Decay (PSD). Chapters feature detailed examples, questions, and problems to help readers solidify their understanding of the concepts and techniques.This is a core reference for scientists and researchers in academic laboratories and pharmaceutical, chemical, and biotech companies. It is also an excellent text for undergraduate or graduate level courses in analytical chemistry, instrumental analysis, biomolecule analysis, and mass spectrometry. Zusammenfassung Even Electron Mass Spectrometry with Biomolecule Applications covers the basic design and theory of the most widely used/traditional mass spectrometers. The text covers the fundamentals of mass spectrometry, along with the basic tools needed for spectral interpretation. Inhaltsverzeichnis Preface xiii Acknowledgments xiv 1 Introduction and Basic Definitions 1 1.1 Definition and Description of Mass Spectrometry 1 1.2 Basic Design of Mass Analyzer Instrumentation 2 1.3 Mass Spectrometry of Protein, Metabolite, and Lipid Biomolecules 5 1.3.1 Proteomics 5 1.3.2 Metabolomics 8 1.3.3 Lipidomics 8 1.4 Fundamental Studies of Biological Compound Interactions 11 1.5 Mass-to-Charge Ratio ( m / z ): How the Mass Spectrometer Separates Ions 13 1.6 Exact Mass versus Nominal Mass 16 1.7 Mass Accuracy and Resolution 18 1.8 High-Resolution Mass Measurements 20 1.9 Rings Plus Double Bonds (r + db) 23 1.10 Nitrogen Rule in Mass Spectrometry 24 1.11 Problems 25 References 27 2 Ionization in Mass Spectrometry 29 2.1 Ionization Techniques and Sources 29 2.2 Electron Ionization (EI) 30 2.3 Chemical Ionization (CI) 32 2.3.1 Positive Chemical Ionization 34 2.3.2 Negative Chemical Ionization 39 2.4 Atmospheric Pressure Chemical Ionization (APCI) 40 2.5 Electrospray Ionization (ESI) 42 2.6 Na...
