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Zusatztext "The findings are brought together in this fine book! which may serve in the future as a model for similar publications on other cell types." (Microbiology Today! November 2001)"Cet ouvrage est donc une tres bonne introduction a l'analyse du genome d'une bacterie." (Sciences Des Aliments - French! No.6! 2001)"a good practical manual" (Biochemie! No.83! 2001)"Two teams of laboratories...analyze thousands of newly discovered bacterial genes to try to discover the function of the 40-60% of them for which that is not known." (SciTech Book News! Vol. 25! No. 4! December 2001) Informationen zum Autor Wolfgang Schumann, Institute of Genetics, University of Bayreuth, Germany S. Dusko Erhlich, Laboratoire de Génétique Microbienne, INRA, Jouy-en-Josas, France Naotake Ogasawara, Graduate School of Biological Sciences, NAIST, Ikoma, Japan Klappentext Functional Analysis of Bacterial Genes: A Practical Manual summarises the principles and the key methods used to analyse the function of genes in bacteria. Written by members of the Bacillus subtilis Functional Analysis Consortium, this book provides a guide to the many approaches for the systematic analysis of gene function.Features include:* In-depth discussion of the theory and application of the methods* A step-by-step practical guide to each method* Experimental examples to indicate expected results* Contact information for suppliers and resource centresWritten by internationally recognised authors, Functional Analysis of Bacterial Genes: A Practical Manual will be a valuable resource to researchers and technicians using functional analysis for many bacterial species, whether in the academic departments of microbiology or genetics, the biotechnology, biomedicine or pharmaceutical industries, or other centres of genomics and bioinformatics."This volume, and the pioneering effort it represents, will be of interest not only to those of us who are specialists in B. subtilis but to all microbiologists and genome scientists who face the formidable task of uncovering the functions of the myriad uncharacterised genes that are emerging from large-scale sequencing." - Richard Losick , The Biological Laboratories, Harvard University, Cambridge, Massachusetts, USA Zusammenfassung This book covers the principles and practice of the functional analysis methods, provides experimental examples to indicate expected results, and offers trouble-shooting tips and notes on time consideration. Inhaltsverzeichnis List of Contributors. Foreword. Preface. Safety. Aims of the Functional Analysis Project. In silico Genome Analysis. Inactivation of Bacillus subtilis Genes Without Known Functions. Analysis of Essential Genes. Transcriptional Analysis of Large Regions of the Bacterial Genome. Useful Methods for Transcriptional Analysis. Northern Blot Analysis with non-RI Detection Systems. Micado, an Integrative Database Dedicated to the Functional Analysis of Bacillus subtilis and Microbial Genomics. Phenotype Responses and Reporter Gene Activity from the Systematic Functional Analysis of Bacillus subtilis Unknown Genes. The Contribution of the EC Consortium to the Two-dimensional Protein Index of Bacillus subtilis. Whole-Cell Fingerprinting - Pyrolysis Mass Spectrometry. Metabolism of Small Molecules and Inorganics. Screening for Genes Involved in Nucleotide and Nitrogen Metabolism. Screening for Growth and lacZ Reporter Gene Induction with Metal Ions and Chelators, Amino Acid Analogs, Antibiotics, Inhibitors of Oxidative Phosphorylation and Miscellaneous Stress Factors. Nucleotide Metabolism. Utilization of Small Carbon-containing Molecules by Bacillus subtilis (S. Fillinger, et al.) . Screening for Gen...
Relazione
"The findings are brought together in this fine book, which may serve in the future as a model for similar publications on other cell types." (Microbiology Today, November 2001)
"Cet ouvrage est donc une tres bonne introduction a l'analyse du genome d'une bacterie." (Sciences Des Aliments - French, No.6, 2001)
"a good practical manual" (Biochemie, No.83, 2001)
"Two teams of laboratories...analyze thousands of newly discovered bacterial genes to try to discover the function of the 40-60% of them for which that is not known." (SciTech Book News, Vol. 25, No. 4, December 2001)
