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This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.
Sommario
1 Membrane Proteins Production for Structural Analysis.- 2 Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies.- 3 Bacterial Expression and Stabilization of GPCRs.- 4 Membrane Protein Production in Escherichia Coli: Overview and Protocols.- 5 Lactococcus Lactis, Recent Developments in Functional Expression of Membrane Proteins.- 6 Over expression of Membrane Proteins in Saccharomyces Cerevisiae for Structural and Functional Studies: a Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid "Flippase" Complex Drs2p/Cdc50p.- 7 Amphipols: a General Introduction and Some Protocols.- 8 New Amphiphiles to Handle Membrane Proteins: "Ménage à Trois" between Chemistry, Physical-Chemistry and Biochemistry.- 9 Building Model Membranes with Lipids and Proteins: Dangers and Challenges.- 10 Analytical Ultracentrifugation and Size Exclusion Chromatography Coupled to Light Scattering for the Characterization of Membrane Proteins in Solution.- 11 Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins.- 12 Micelles, Bicelles, Amphipols, Nano discs, Liposomes or Intact Cells: the Hitchhiker's Guide to the Membrane Protein Study by NMR.- 13 Foundations of Bio molecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations and Free Energy Calculations of Membrane Proteins.- 14 Structural Studies of TSPO, a Mitochondrial Membrane Protein.
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Dr. Isabelle Mus-Veteau is a biochemist and biophysicist specialist in membrane protein characterization. She obtained her PhD in microbiology and cell biology at the University of Marseille in France. She has held a French National Centre for Scientific Research (CNRS) tenure position at the Institute of Molecular and Cellular Pharmacology (IPMC, Sophia Anti polis near Nice, France), where she supervises projects on the characterization of the Hedgehog receptor Patched. She was a member of the CNRS tenure position recruitment committee and is currently a member of the executive committees of the French Biophysical Society and of the Membrane Group Society. She organized two international summer schools on membrane protein production for structural analysis and two international congresses on membrane biophysics.
Riassunto
This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.
