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Klappentext Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Structural ApproachesReconstitution of Heterogeneous SystemsIntracellular Methods and AssaysGenome-Wide Analyses of Two-Component Systems Presents detailed protocols Includes troubleshooting tips Zusammenfassung Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This title features sections including: Structural Approaches; Reconstitution of Heterogeneous Systems; and! Genome-Wide Analyses of Two-Component Systems. It presents protocols and includes troubleshooting tips. Inhaltsverzeichnis Section I: Structural Approaches [1]: The PICM Chemical Scanning Method for Identifying Domain-Domain and Protein-Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis [2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis [3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases [4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR [5]: TonB/TolA Amino-Terminal Domain Modeling [6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques [7]: The Design and Development of Tar-EnvZ Chimeric Receptors [8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli [9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis [10]: A Temperature-Sensing Histidine Kinase-Function, Genetics, and Membrane Topology [11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules Section II: Reconstitution of Heterogeneous Systems [12]: Liposome-Mediated Assembly of Receptor Signaling Complexes [13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines [14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers [15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases [16]: Genetic Dissection of Signaling Through the Rcs Phosphorelay Section III: Intracellular Methods and Assays [17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis [18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes [19]: In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays [20]: A "Bucket of Light? for Viewing Bacterial Colonies in Soft Agar [21]: Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors [22]: Single-Cell Analysis of Gene Expression by Fluorescence Microscopy Section IV: Genome-Wide Analyses of Two-Component Systems [23]: Two-Component Systems of Mycobacterium tuberculosis-Structure-Based Approaches [24]: Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus [25]: Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling [26]: Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays [27]: Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques Subject Index Author ...
