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Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
List of contents
I Methods.- Housekeeping Genes: A Gold Standard?.- The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia.- Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach.- Measuring Genome Sizes by Absolute Quantification.- II Applications.- Regulation and Development.- Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I.- Quantification of ?Rec-? J? Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation.- Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR.- Quantification of Ikaros Splice Variants by Real-Time PCR.- Methods to Quantify Cytokine Gene Expression by Real-Time PCR.- Oncology.- Profiling Breast Cancer Using Real-Time Quantitative PCR.- HER-2/neu Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue.- Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer.- A Rapid Cycle Real-Time Quantitative (?-globin PCR for Quantification of Human DNAin Feces.- Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycler(TM).- Real-Time Quantification of the AML Rearrangements, AML1-ETO and TEL-AML1, in Acute Leukemia.- Genetics.- Rapid Detection of Gene Duplications in Charcot-Marie-Tooth 1A Disease by SNPGenotyping Using Real-Time PCR.- Cytochrome P450 2D6 Deletion Genotyping Using Derivative Curve Analysis on the LightCycler.- Trisomy 21 Detected by SNP Allele Ratios.- Quantitative Chimerism Analysis by Allele-Specific Real-Time PCR of a 10 bpInsertion/Deletion Polymorphism within the Promotor Region of Factor VIIc.- Microbiology.- Detection of SARS-Coronavirus in the LightCycler by 5?-Nuclease Real-Time RT-PCR.- Normalized Quantitative Rapid-Cycle Real-Time PCR for the Assessment of CMV-DNA Copies.
