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This detailed volume provides practical guidance on techniques in plant-virus interaction research, from targeting specific molecular interactions within the virus-host interactome to the identification of the complete virus-host protein-protein interaction network. After chapters on acquiring the necessary molecular tools, the book continues with biochemical and genetic approaches to confirming protein-protein interactions both in vivo and in vitro, procedures and protocols for assessing replication, translation, viral genome movement, and insect transmission, as well as techniques for detecting multiple molecular interactions between the host and the virus and monitoring immune hubs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Plant-Virus Interactions serves as a valuable resource for understanding the protein-protein interaction network between the virus and the host, crucial for comprehending the life cycle of a virus and for developing strategies for broad-spectrum and long-lasting resistance against viral infections.
List of contents
Computational Pipeline for Detection of Plant RNA Viruses Using High-Throughput Sequencing.- Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons.- Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs).- Construction of Geminivirus Infectious Clones for Agroinoculation into Plants.- Agroinoculation of Geminiviral Infectious Clones into Plants.- Begomovirus Inoculation in Arabidopsis and Cassava.- Absolute and Relative Quantification of Single-Stranded Positive Sense RNA Virus from Plant Tissue.- Quantification of Virion-Sense and Complementary-Sense DNA Strands of Circular Single-Stranded DNA Viruses.- Replication Assay of Begomovirus in Arabidopsis Protoplasts.- Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye.- Ribosome Profiling of Plants.- Experimental Transmission of Plant Viruses by Aphids or Whiteflies.- Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana.- Protein-Protein Interaction via Two-Hybrid Assay in Yeast.- Bimolecular Fluorescence Complementation (BIFC) in Host-Virus Interactions.- Co-Immunoprecipitation Assays to Detect Protein-Protein Interactions.- Split-Luciferase Complementation Imaging Assay in Virus-Plant Interactions.- The Split-Luciferase Complementation Assay to Detect and Quantify Protein-Protein Interactions In Planta.- TurboID-Based Proximity Labeling: A Method to Decipher Protein-Protein Interactions in Plants.- Virus-Induced Heritable Gene Editing in Plants.- Immunoprecipitation Followed by Mass Spectrometry: An Approach for Identifying Host-Viral Protein-Protein Interactions.- In Situ Protein Microarray for Identifying the Geminivirus-Arabidopsis Interactome.
