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Zusatztext "This book is an ideal practical reference work for the clinical scientist and is recommended for all laboratories in hospitals and research institutions which are actively involved in analytical work."--Clinical Laboratory International! June 2000 Informationen zum Autor J. Lunec and H. R. Griffiths are the authors of Measuring in vivo Oxidative Damage: A Practical Approach, published by Wiley. Klappentext Seit ungefähr 15 Jahren weiß man, daß freie Sauerstoffradikale eine Schlüsselrolle bei der Entstehung bestimmter Krankheiten (Arteriosklerose, chronische Entzündungen) spielen. Dieses Handbuch der Methodologie der biologischen Analytik auf dem Gebiet der oxidativen Schädigungen richtet sich an klinische Wissenschaftler, die eine geeignete in-vivo-Methode suchen. Übersichtlich in fünf Teile unterteilt, ist das Buch ein praktischer Begleiter im Laboralltag. (03/00) Zusammenfassung Oxygen free radicals have been implicated in a number of disease processes. This book provides a comprehensive guide for methodology in the analysis of biological samples. It is designed to help the clinical scientist evaluate the best method for their particular problem. Inhaltsverzeichnis CHROMATOGRAPHIC PROCEDURES. Lipid Peroxidation Determination by HPLC (R. Bevan). Measurement of Oxidized Bases in DNA and Biological Fluids by Gas Chromatography Coupled to Mass Spectrometry (T. Douki, et al.). The Measurement of Protein Oxidation by HPLC (H. Griffiths, et al.). MEASUREMENT OF 8-OXO DEOXYGUANOSINE. Measurement of 8-Oxo-2' -deoxyguanosine in Cellular DNA by High Performance Liquid Chromatography-Electrochemical Detection (M. Evans). Immunochemical Detection of 8-Oxodeoxyguanosine in DNA (M. Cooke & K. Herbert). Urinary Measurement of 8-OxodG (8-Oxo-2' -deoxyguanosine) (H. Poulsen, et al.). CELLULAR-BASED METHODS. Measurement of Oxidative DNA Damage Using the Comet Assay (A. Collins). Measuring Oxidative DNA Damage by Alkaline Elution (M. Pflaum & B. Epe). MOLECULAR-BASED ASSAYS. A ¯32P-Postlabelling Protocol to Measure Oxidative DNA Damage (G. Jones & M. Weinfeld). Mapping Reactive Oxygen-Induced DNA Damage at Nucleotide Resolution (H. Rodriguez & S. Akman). ANTIOXIDANT ACTIVITY. Measurements of Plasma Antioxidant Activity (S. Maxwell). Index....
