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This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.
List of contents
Strategies for Multiplexed Biosensor Imaging to Study Intracellular Signaling Networks.- Six-color Confocal Immunofluorescence Microscopy with 4-Laser Lines.- Multiplexed Imaging of Posttranslational Modifications of Endogenous Proteins in Live Cells.-Multiplex Imaging of Rho GTPase Activities in Living Cell.- Multicolor Localization-Based Super Resolution Microscopy.- Multiplexed Tissue Tomography.- Multicolor 3D Confocal Imaging of Thick Tissue Section.- Multiphoton Deep-Tissue Imaging of Micrometastases and Disseminated Cancer Cells Using Conjugates of Quantum Dots and Single-Domain Antibodies.- Multiplexed Imaging for immune profiling on Human FFPE Material.- Method for Multiplexed Dynamic Intravital Multi-Photon Imaging.- Fourier Multiplexed Fluorescence Lifetime Imaging.- Bimolecular Fluorescence Complementation (BiFC) and Multiplexed Imaging of Protein-Protein Interactions in Human Living Cells.- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging under Adverse Optical Conditions.- Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells.- Nanoparticles for In Vivo Lifetime Multiplexed Imaging.- Versatile on-Demand Fluorescent Labeling of Fusion Proteins using Fluorescence-Activating and Absorption-Shifting Tag (FAST).- UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry.- Multi-Modal Approach for Cancer Cells Investigation.- Multiplexed Fourier Transform Infrared and Raman Imaging.- Multiplexed Imaging Mass Spectrometry of Histological Staining, N-glycan and Extracellular Matrix from One Tissue Section: A Tool for Fibrosis Research.- Multiplexed Raman Imaging in Tissues and Living Organisms.
