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Informationen zum Autor Botros Rizk , MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USA Markus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany Klappentext Compiled by two experts in Reproductive Medicine! with contributions from internationally respected specialists! this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic! it details what needs to be prepared in advance! what needs to be prepared earlier the same day! and what steps to take before! during! and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues. Zusammenfassung Compiled by two experts in Reproductive Medicine, with contributions from internationally respected specialists, this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic, it details what needs to be prepared in advance, what needs to be prepared earlier the same day, and what steps to take before, during, and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues. Inhaltsverzeichnis LABORATORY PROCEDURES 1 The documentation system Markus Montag and Lars Johansson 2 Culture Media Techniques Lars Johansson 3 IVF incubator handling Jason E Swain and Alex Lagunov 4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC) Markus Montag 5 Semen collection and sample reception Carin Huyser 6 Spermiogramm, Makler chamber, Neubauer chamber, CASA Verena Nordhoff 7 Sperm viability testing Markus Montag 8 Sperm preparation – Swim up, density gradient, migration chamber, zeta method Thomas Ebner 9 Sperm selection using hyaluronan binding Denny Sakkas 10 IVF - Insemination Markus Montag 11 ICSI Thomas Freour 12 Oocyte in vitro maturation – Isolation of COCs and culture for maturation Mariabeatrice Dal Canto and Giovanni Coticchio 13 Artificial oocyte activation Markus Montag 14 Cryopreservation - Sperm TESE and MESA Borut Kovacic 15 Vitrification – Oocytes, 2PN, Embryos, Blastocysts Ana Cobo and Aila Coello 16 Slow freezing: principles, oocytes, 2PN, embryos, blastocysts Etienne Van den Abbeel 17 Oocyte selection Basak Balaban 18 Zygote selection Martin Greuner 19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6 Thorir Hardarson and Julius Hreinsson 20 Time-lapse imaging Cristina Hickman 21 Assisted hatching Maria De Geyter and Christian De Geyter 22 Zona manipulation at various embryo stages: Mechanical / acidic medium Marius Meintjes 23 Biopsy: polar body, blastomere, trophectoderm Hakan Kadir Yelke and Semra Kahraman 24 Sampling of cells after biopsy for aCGH Barry Behr 25 Embryo transfer – standard and ultrasound guided Bettina Toth 26 Hygenic standards in the IVF laboratory Vera Baukloh 27 Quality control of IVF laboratory supplies – Mouse Embryo Assa...
