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Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA. Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes. The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.
Summary
Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA. Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes. The second edition of
Pancreatic Cancer: Methods and Protocols
provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It covers topics from
in-vitro
cell cultures to
in-vivo
mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful
Methods in Molecular Biology™
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.
Additional text
From the reviews of the second edition:
“This book describes methods and techniques that are used in pancreatic cancer research. … It will be most useful for postdoctoral researchers and novice principal investigators, as well as any investigators who want to focus on pancreatic cancer research, particularly if they are looking for a technique to fit a specific need. … It is a useful reference and go-to guide for exploring new protocols and techniques. It is also handy for seeing how an existing technique might be altered slightly to suit another need.” (Naomi Akagi, Doody’s Book Reviews, September, 2013)
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From the reviews of the second edition:
"This book describes methods and techniques that are used in pancreatic cancer research. ... It will be most useful for postdoctoral researchers and novice principal investigators, as well as any investigators who want to focus on pancreatic cancer research, particularly if they are looking for a technique to fit a specific need. ... It is a useful reference and go-to guide for exploring new protocols and techniques. It is also handy for seeing how an existing technique might be altered slightly to suit another need." (Naomi Akagi, Doody's Book Reviews, September, 2013)
