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This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis. Chapters in BacterialCell Wall Homeostasis cover a variety of subjects, such as: modernmicroscopy techniques and other biophysical methods used to characterize thesubcellular structure of the bacterial cell wall; high-throughput approachesthat can be used to identify all the genes and proteins that participate in thecorrect functioning of an organism's cell wall; protocols for assayingindividual gene products for specific cell wall functions or identify chemicalswith inhibitory activity against the cell wall; and methods for analyzing the non-proteincomponents of the cell wall and the increasing use of computational approachesfor predicting and modeling cell wall related functions and processes. Writtenin the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary material and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Thoroughand cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocolsemphasizes the diversity of the research taking place in bacterial cell wallhomeostasis, and explains how the integration of information from acrossmultiple disciplines is going to be essential if a holistic understanding ofthis important process is to be obtained.
Summary
This volume brings
together the most widely used and important protocols currently being employed
in researching and understanding bacterial cell wall homeostasis. Chapters in
Bacterial
Cell Wall Homeostasis
cover a variety of subjects, such as: modern
microscopy techniques and other biophysical methods used to characterize the
subcellular structure of the bacterial cell wall; high-throughput approaches
that can be used to identify all the genes and proteins that participate in the
correct functioning of an organism’s cell wall; protocols for assaying
individual gene products for specific cell wall functions or identify chemicals
with inhibitory activity against the cell wall; and methods for analyzing the non-protein
components of the cell wall and the increasing use of computational approaches
for predicting and modeling cell wall related functions and processes. Written
in the highly successful
Methods in Molecular Biology
series format,
chapters include introduction to their respective topics, lists of the
necessary material and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and cutting-edge,
Bacterial Cell Wall Homeostasis: Methods and Protocols
emphasizes the diversity of the research taking place in bacterial cell wall
homeostasis, and explains how the integration of information from across
multiple disciplines is going to be essential if a holistic understanding of
this important process is to be obtained.
