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The developing new field of proteomics holds significant promise for a deeper understanding of complicated biological systems, as well as for novel approaches to the cure of disease. In the Handbook of Proteomic Methods, P. Michael Conn has assembled a panel of well-recognized experts to describe a wide range of powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. These readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, post-translational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology. Each fully tested protocol is described in step-by-step detail, complete with tricks of the trade and hints on avoiding pitfalls. The detail of description exceeds that usually found in primary publications.
Wide-ranging and practical, the Handbook of Proteomic Methods offers scientists entering, or already active in, the field a fully referenced compendium of easy-to-use proteomic methods-each reflecting the power and promise of proteomics in illuminating complex biological systems and in promoting the development of novel drugs for the amelioration of disease.
Sommario
"Part I. General Techniques
Proteomics and the Molecular Biologist
Paul R. Graves and Timothy A. J. Haystead
Protein Identification from 2-D Gels Using In Vitro Transcription Translation Products
Nathalie Norais, Renzo Nogarotto, Emilia Tiziana Iacobini, Ignazio Garaguso, Renata Grifantini, Giuliano Galli, and Guido Grandi
Selective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive Degradation
Akira Tsugita
Means of Hydrolyzing Proteins Isolated upon ProteinChip® Array Surfaces: Chemical and Enzymatic Approaches
Shanhua Lin, Ning Tang, and Scot R. Weinberger
A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels
Jules A. Westbrook and Michael J. Dunn
Qualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Technology
Michael P. Washburn and David M. Schieltz
Di- and Tri-Chromatic Fluorescence Detection on Western Blots
Karen J. Martin and Wayne F. Patton
Multiplexed Proteomics: Fluorescence Detection of Protein Differences by Two-Dimensional Gel Electrophoresis and Computer-Based Differential Display
Birte Schulenberg and Wayne F. Patton
A Strategy for Characterizing Antibody/Antigen Interactions Using ProteinChip® Arrays
Alexandra Huhalov, Daniel I. R. Spencer, and Kerry A. Chester
Stable Isotope Labeling with Amino Acids as an Aid to Protein Identification in Peptide Mass Fingerprinting
Robert J. Beynon
The Use of 18O Labeling as a Tool for Proteomic Applications
Ian I. Stewart, Ty Thomson, Daniel Figeys, and Henry S. Duewel
Automated Nanoflow Liquid Chromatography/Tandem Mass Spectrometric Identification of Liver Mitochondrial Proteins
Bart Devreese, Frank Vanrobaeys, Elke Lecocq, Joe l Smet, Rudy Van Coster, and Jozef Van Beeumen
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Riassunto
A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.
Testo aggiuntivo
"...covers recent advances and also acts as a manual of well-practiced techniques. This volume should appeal particularly to the uninitiated but contains several articles of interest to more experienced practitioners...As well as being an excellent guide, this volume in its entirety makes a strong case for the advantages in the application of these post-genomic technologies" - Rapid Communications in Mass Spectrometry
"Handbook of Proteomic Methods includes a wide range of techniques for identifying and analyzing proteins expressed in cells. As many other Humana Press method books, this is another must-have for large academic libraries supporting research in complex biological systems and drug development." -E-STREAMS
"This is a very well produced volume with excellent diagrams and reproduction of difficult images such as 2-D gels...deserves a place on the proteomic researchers' bookshelf." - Chromatographia
