Ulteriori informazioni
This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.
Sommario
Evaluation of prototype CE-MS interfaces.- Capillary Electrophoresis-Mass Spectrometry (CE-MS) by Sheath-Flow Nanospray interface and its use in biopharmaceutical applications.- Protocol for etching bare-fused silica capillaries for sheathless capillary electrophoresis-mass spectrometry coupling.- Microfabricated Liquid Junction Capillary Electrophoresis-Mass Spectrometry Interface.- Successive Multiple Ionic-Polymer Layer Coatings for intact protein analysis by Capillary Zone Electrophoresis - Mass Spectrometry - Application to Hemoglobin Analysis.- On-line Immobilized Enzyme Microreactor Capillary Zone Electrophoresis-Mass Spectrometry for Peptide Mapping.- Bottom up analysis of proteins by peptide mass fingerprinting with tCITP-CZE-ESI-TOF MS after tryptic digest.- Top-down proteomics by capillary zone electrophoresis-tandem mass spectrometry for large-scale characterization of proteoforms in complex samples.- Assessment of macro- and microheterogeneity of monoclonal antibodies using Capillary Zone Electrophoresis hyphenated with Mass Spectrometry.- High Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS.- Capillary Zone Electrophoresis-Electrospray Ionization Tandem Mass Spectrometry for Total Analysis of Chondroitin/Dermatan Sulfate Oligosaccharides.- In-Source Fragmentation for Identification of Compounds by CE-ESI-TOF in Human Plasma. L-Proline as case study.- Assessing the energy status of low numbers of mammalian cells by capillary electrophoresis-mass spectrometry.- Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis.- Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction coupled to Capillary Electrophoresis-Tandem Mass Spectrometry.- CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis.
