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Soil sterilization in screenhouse experiments eliminates the effect of soil pathogenic microbes where reaction of plants to artificial inoculum is being investigated. The objective of this study was to evaluate the effect of soil sterilization on 10 common bean genotypes reaction to the anthracnose pathogen under screenhouse conditions. The experiments were conducted at KARI-Kakamega. Bean seedlings were planted in both sterile and non sterile potted soils and inoculated 10 days after germination. Data on growth parameters, disease incidence and severity (CIAT 1-9 scale) was collected. Data was analyzed using the SAS statistical software (version 9.1). Of the 10 genotypes, 5 showed high levels of resistance to C. lindemuthianum of 3 while the other five genotypes were tolerant. Soil sterilization altered the performance of the plants, but did not significantly affect their reaction to anthracnose. Notwithstanding, non-sterile soil should be used alongside sterile soil for screenhouse experiments when evaluating for anthracnose disease.
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The Author is the holder of a Master of Science degree in Crop Protection from Masinde Muliro University of Science and Technology. He attained his Bachelor of Science Degree in Agricultural Education and Extension from Egerton University. He is currently enrolled for a PHD in Sustainable Agricultural Systems from MMUST.
