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Klappentext Combining computational and theoretical techniques with advanced chromatographic methods offers a powerful tool for quantitatively determining the molecular interactions taking place in separation and biological systems . Written by the author of HPLC: A Practical Guide (RSC, 1999), the book uses computational models to examine the interactions of analytes, including many biological molecules, in chromatographic systems and extends these models to the behaviour of proteins in of biological reactions per se.Various software packages are reviewed, and most computations can be performed on a standard PC using accessible software. Consideration is given to a variety of chromatographic techniques and strategies for high-sensitivity detection are presented.The first book of its kind, it will inspire readers to explore the possibilities of combining these techniques in their own work, whether at an industrial or academic level. Zusammenfassung Written by the author of “HPLC: A Practical Guide” (RSC, 1999),this book presents the possibilities for characterising biological applications by combining analytical and computational chemistries. Inhaltsverzeichnis Preface; Introduction; Basic Concept of Moelcuar Interaction Energy Values; Design Model-Phases in Chromatography; Retention in Gas Chromatography; Retention in Normal-Phase Liquid Chromatography; Retention in Reversed-Phase Liquid Chromatography; Retention in Ion-Exchange Liquid Chromatography; Enantiomer Recognition; Human Serum Albumin-Drug Binding Affinity Based on Liquid Chromatography; Affinity and Reactivity of Proteins; Mechanism of Highly Sensitive Detection; Appendix; Subject Index
