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This detailed volume provides a single, valuable reference source formethods that definitively identify and accurately quantify apoptosis. The bookbegins with common methods utilized to detect and quantitate apoptosis, as wellas apoptosis signaling pathways in toxicological and other related research. Itcontinues with multi-parametric and phased apoptosis assays for detecting earlyand late apoptosis or distinguishing apoptosis from necrosis and autophagy.Subsequent chapters focus on recent advances in real time and high-throughputassays that detect and quantitate apoptosis and apoptosis signaling pathways.Final chapters focus on recent developments in preclinical anticancertherapeutics targeting apoptosis. Written for the Methods in Pharmacologyand Toxicology series, chapters feature step-by-step descriptions of themethodologies, as well as expert tips and implementation advice.
Vital and authoritative, Apoptosis Methods inToxicology serves novice scientists as well as experts,utilizing a range of instruments from common laboratory equipment to high-endexpensive and automated machinery capable of performing real time apoptoticmeasurements.
Table des matières
An Overview ofApoptosis Methods in Toxicological Research: Recent Updates.- Detection ofApoptosis: From Bench Side to Clinical Practice.- Determining the Extent ofToxicant-Induced Apoptosis Using Concurrent Phased Apoptosis Assays.- Measurementof Apoptosis by Multiparametric Flow Cytometry.- Detecting Apoptosis,Autophagy, and Necrosis.- A Low-Cost Method for Tracking the Induction ofApoptosis Using FRET-Based Activity Sensors in Suspension Cells.- FRET-BasedMeasurement of Apoptotic Caspase Activities by High-Throughput Screening FlowCytometry.- Automated Ratio Imaging Using Nuclear-Targeted FretProbe-Expressing Cells for Apoptosis Detection.- Antibody-Based ProteomicAnalysis of Apoptosis Signaling.- Novel Electrochemical Biosensor for ApoptosisEvaluation.- Microplate-Based Whole Zebrafish Caspase 3/7 Assay for ScreeningSmall Molecule Compounds.- Targeting Cancer Cell Death with Small-MoleculeAgents for Potential Therapeutics.- Liposomes in Apoptosis Induction and CancerTherapy.
Résumé
This detailed volume provides a single, valuable reference source formethods that definitively identify and accurately quantify apoptosis. Itcontinues with multi-parametric and phased apoptosis assays for detecting earlyand late apoptosis or distinguishing apoptosis from necrosis and autophagy.
