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The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
Table des matières
Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues.- Detecting p53 Isoforms at Protein Level.- Autophagy, Senescence and Apoptosis.- p53 and Cell Cycle Effects after DNA Damage.- p53 Ubiquitination and Proteasomal Degradation.-Identification of p53 in Mitochondria.-Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.-Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization.- Determine the Effect of p53 on Chemosensitivity.-Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells.- Mutant p53 in Cell Adhesion and Motility.- Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication.- Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts.- p53 Actions on MicroRNA Expression and Maturation Pathway.- Isolation and Characterization of Murine Multipotent Lung Stem Cells.- Generation of p53 Knock-down Cell Lines.-ChIP for Identification of p53 Responsive DNA Promoters.- ChIP-on-chip to Identify Mutant p53 Targets.- ChIP Sequencing to Identify p53 Targets.- The p53-Mdm2 Loop: A Critical Juncture of Stress Response.
Résumé
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition.
