En savoir plus
Zusatztext it is more than successful, and most, if not all, who dip into it will be rewarded by learning things they did not know, or possibly had forgotten...[..] a book that will surely find its place in every crystallographic lab whether protein or macromolecular. Informationen zum Autor Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins.Jane Skelly has widespread experience in the expression and crystallisation of macromolecules.Both editors lecture undergraduates in structural biology. Klappentext Macromolecular crystallography is the study of macromolecules using X-ray crystallographic techniques to determine their molecular structure. This title synthesises contributions from a team of internationally recognized leaders, offering chapters on conventional and high-throughput methods. Zusammenfassung Macromolecular crystallography is the study of macromolecules using X-ray crystallographic techniques to determine their molecular structure. This title synthesises contributions from a team of internationally recognized leaders, offering chapters on conventional and high-throughput methods. Inhaltsverzeichnis 1: Skelly, Sohi and Batuwangala: Classical Cloning, Expression and Purification 2: Owens, Nettleship, Berrow, Stuart and Stammers: High-Throughput Cloning, Expression and Purification 3: Chayen: Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation 4: Abdel-Meguid, Jeruzalmi and Sanderson: First Analysis of Macromolecular Crystals 5: Sanderson: In-House Macromolecular Data Collection 6: Abdel-Meguid: Solving the Phase Problem Using Isomorphous Replacement 7: Delarue: Molecular Replacement Techniques for High-Throughput Structure Determination 8: Murthy: MAD Phasing 9: Weeks and Furey: Application of Direct Methods to Macromolecular Structure Solution 10: Abrahams: Phase Refinement through Density Modification 11: Morris, Perrakis and Lamzin: Getting a Macromolecular Model: Model Building, Refinement and Validation 12: Wasserman, Smith, D'Amico, Koss, Morisco, and Burley: High-Throughput Crystallographic Data Collection at Synchrotrons 13: Carson: Electron Density Fitting and Structure Validation 14: Masquida, Francois, Werner and Westhof: RNA Crystallogenesis 15: Sohi and Laponogov: Crystallography in the Study of Protein-DNA Interaction 16: Fry, Abrescia and Stuart: Virus Crystallography 17: Abdel-Meguid: Macromolecular Crystallography in Drug Design ...
