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Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips.
Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource.
List of contents
Evolution of the Wnt Pathways.- Dictyostelium.- Dictyostelium Development: A Prototypic Wnt Pathway?.- Monitoring Patterns of Gene Expression in Dictyostelium by ?-galacotsidase Staining.- Use of the Dictyostelium Stalk Cell Assay to Monitor GSK-3 Regulation.- Cnidarians.- Wnt Signaling in Cnidarians.- Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos.- Detection of Expression Patterns in Hydra Pattern Formation.- C. Elegans.- Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development.- Wnt Signaling During Caenorhabditis elegans Embryonic Development.- Drosophila.- Function of the Wingless Signaling Pathway in Drosophila.- Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster.- Wingless Signaling in Drosophila Eye Development.- High-Throughput RNAi Screen in Drosophila.- Sea Urchin.- Wnt Signaling in the Early Sea Urchin Embryo.- Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos.- Functional Analysis of Wnt Signaling in the Early Sea Urchin Embryo Using mRNA Microinjection.- Zebrafish.- Wnt Signaling Mediates Diverse Developmental Processes in Zebrafish.- Determination of mRNA and Protein Expression Patterns in Zebrafish.- Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches.- Neural Patterning and CNS Functions of Wnt in Zebrafish.- Xenopus.- Studying Wnt Signaling in Xenopus.- Analysis of Gene Expression in Xenopus Embryos.- Detection of Nuclear ?-catenin in Xenopus Embryos.- Transgenic Reporter Tools Tracing Endogenous Canonical Wnt Signaling in Xenopus.- Gain-of-Function and Loss-of-Function Strategies in Xenopus.- How The Mother Can Help: Studying Maternal Wnt Signaling by Anti-sense-mediated Depletion ofMaternal mRNAs and the Host Transfer Technique.- Inducible Gene Expression in Transient Transgenic Xenopus Embryos.- Wnt-Frizzled Interactions in Xenopus.- Dorsal Axis Duplication as a Functional Readout for Wnt Activity.- Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo.- Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula.
Summary
Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips.
Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource.
