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This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field.
List of contents
Scarless ssDNA Recombineering of Phage in the Lysogenic State.- Lambda red Recombineering of Bacteriophage in the Lysogenic State.- Recombineering Mediated Genome Editing in Burkholderiales Strains.- High-Efficiency Multi-Site Genomic Editing (HEMSE) Made Easy.- Creation of Markerless Genome Modifications in a Non-Model Bacterium by Fluorescence-Aided Recombineering.- Plant Gene Modification by BAC Recombineering.- Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling.- Genome Editing in Klebsiella pneumoniae using CRISPR/Cas9 Technology.- Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System.- Recombineering in Staphylococcus aureus.- Genome Editing Methods for Bacillus subtilis.- Highly Efficient Genome Editing in Clostridium difficile using the CRISPR-Cpf1 System.- Genome Editing of Corynebacterium glutamicum using CRISPR-Cpf1 System.- CRISPR/Cas-Mediated Genome Editing of Streptomyces.- Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum.- Genome Editing with Cas9 in Lactobacilli.
