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This detailed book focuses on cutting-edge methods for Parkinson's disease (PD) research, such as the analysis of the prion-like properties of -synuclein, mitochondrial functions related to the PINK1-Parkin pathway/CHCHD2, the endolysosome pathway related to LRRK2, VPS35, and ATP13A2 using cultured cells (including patient iPS cells), deep brain stimulation therapy, classic mitochondrial toxins related to PD, and genetic associations and screenings using mammalian and invertebrate genetic models of PD. The collection intends to serve as an introductory protocol book for basic research on PD pathogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Experimental Models of Parkinson's Disease is an ideal guide for researchers continuing to work toward making PD risk predictable and surmountable.
List of contents
-Synuclein Seeding Assay Using RT-QuIC.- Electron-Microscopic Analysis of -Synuclein Fibrils.- -Synuclein Seeding Assay Using Cultured Cells.- Analysis of -Synuclein in Exosomes.- Measurement of GCase Activity in Cultured Cells.- Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells.- Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement.- Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells.- Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells.- Generation of Mitochondrial Toxin Rodent Models of Parkinson's Disease Using 6-OHDA, MPTP, and Rotenone.- Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson's Disease.- -Synuclein Propagation Mouse Models of Parkinson's Disease.- Common Marmoset Model of -Synuclein Propagation.- Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections.-Deep Brain Stimulation Using Animal Models of Parkinson's Disease.- Assessment of Cytotoxicity of -Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy.- The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons.- Analysis of Dopaminergic Functions in Drosophila.- Evaluation of Mitochondrial Function and Morphology in Drosophila.- Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons.
