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This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.
List of contents
Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays.- Quantification of Integrin Activation and Ligation in Adherent Cells.- Multi-parametric Analysis of Focal Adhesions in Bi-Dimensional Substrates.- Focal Adhesion Isolation Assay using ECM Coated Magnetic Beads.- Functional Integrin Regulation through Interactions with Tetraspanin CD9.- Proximity-dependent Biotinylation (BioID) of Integrin Interaction Partners.- Analyzing the Integrin Adhesome by in situ Proximity Ligation Assay.- Single-Protein Tracking to Study Protein Interactions during integrin-based Migration.- Biochemical Characterization of the Integrin Interactome.- Network Analysis of Integrin Adhesion Complexes.- Surface Patterning for the Control of Receptor Clustering and Molecular Forces of Integrin-mediated Adhesions.- Dynamics and Physics of Integrin Activation in Tumour Cells by Nano-Sized Extracellular Ligands andElectromagnetic Fields.- Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation.- Quantitative Analysis of Integrin Trafficking.- Methods to Study Integrin Functions on Exosomes.- Functional Bioinformatics Analyses of the Matrisome and Integrin Adhesome.- Quantifying Polarized Extracellular Matrix Secretion in Cultured Endothelial Cells.
