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This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors.
List of contents
Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs.- Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine.- Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue.- Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA.- 2 -C,4 -C-Ethyleneoxy-Bridged 2 -Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability.- Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids.- Synthesis of Nucleobase-Functionalized Morpholino Monomers.- Synthesis and Application of LKGammaT Peptide Nucleic Acids.- Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids.- Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site.- Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids.- Labeling Peptide Nucleic Acids with Indium-111.- Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet.- Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates.- Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis.- FRET Assay for Ligands Targeting the Bacterial A-Site RNA.- The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons.- Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.- Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation.
