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Capillary electrophoresis-mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this book, the unique features of CE-MS for metabolomics studies are highlighted including CE separation modes, capillary coatings and practical aspects of CE-MS coupling alongside a comprehensive overview of recent technological developments and applications.
CE-MS can be considered a relatively new technique in the field of metabolomics and it is therefore important to inform the scientific community about the possibilities of advanced CE-MS approaches for metabolomics studies. This book outlines the potential of this technique for researchers working in metabolomics, bioanalytics and biomarker analysis.
List of contents
Capillary Electrophoresis-Mass Spectrometry for Metabolomics - From Metabolite Analysis to Metabolic Profiling; CE-MS Workflows for Metabolomics: A Focus on Sample Preparation; Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries for Metabolic Profiling of Biological Samples; Capillary Electrophoresis-Mass Spectrometry for Metabolomics Using New Interfacing Designs; Stacking or On-line Sample Concentration in CE-MS for Metabolomics; On-line Solid-phase Extraction Capillary Electrophoresis-Mass Spectrometry and Data Analysis Approaches for Metabolomics; CE-MS for Anionic and Cationic Metabolic Profiling: System Optimization and Applications; CE-MS for Metabolomics: A Comparison with Other Techniques; Potential of CE-MS for Chiral Metabolic Profiling; Single-cell Metabolomics with Capillary Electrophoresis-Mass Spectrometry; Capillary Electrophoresis-Mass Spectrometry for Lipid Analysis; Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry: A Robust Platform for High Throughput Metabolite Profiling with Quality Assurance; Subject Index
About the author
Strongly believing in the power of analytical technology to contribute to a better understanding of biochemical mechanisms underlying diseases, Rawi Ramautar studied both Pharmacochemistry and Analytical Sciences at Vrije Universiteit of Amsterdam in order to have the right background for a PhD training in this direction. In 2010, he completed his PhD on the development of capillary electrophoresis-mass spectrometry methods for metabolomics at the Utrecht University. Intrigued by metabolomics for disease prediction and diagnosis, Rawi switched to the Leiden University Medical Center to broaden his horizon on this topic. In 2013 and 2017, Rawi Ramautar received the highly prestigious Veni and Vidi personal grants, respectively, from the Netherlands Organisation for Scientific Research. Currently, he is a Principal Investigator (tenured) at the Leiden Academic Center for Drug Research of the Leiden University where his group is developing microscale analytical workflows for volume-restricted biomedical problems.
Summary
In this book, the unique features of CE-MS for metabolomics studies are highlighted and a comprehensive overview of recent technological developments is given at a level suitable for researchers working in metabolomics, bioanalytics and biomarker analysis.
