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The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level. Investigation of the species diversity is required to determine molecular relatedness of isolates for epidemiological studies. Methods for molecular epidemiology of microorganisms must be highly reproducible and provide effective discrimination of epidemiologically unrelated strains. A wide range of techniques has been applied to the investigation of outbreaks of tra- missible disease, and these have been critical in unraveling the route of spread of pat- gens for humans, animals, and plants. The choice of a molecular method will depend on the type of questions to be addressed, on the degree of genetic diversity of the species to be analyzed, and on the mechanisms responsible for generation of the diversity. The applications of molecular methods, singly or in combination, have greatly contributed in the past two decades to basic microbial science and public health control strategies. Molecular Epidemiology of Microorganisms: Methods and Protocols brings together a series of methods-based chapters with examples of application to some of the most important microbes. Both traditional and novel techniques are described, and the type of information that can be expected to be obtained by their application is indicated.
List of contents
Microbial Molecular Epidemiology: An Overview.- Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens.- Plasmid Replicon Typing.- The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms.- Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborne Pathogens.- Pulsed-Field Gel Electrophoresis for Molecular Epidemiology of Food Pathogens.- Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance.- Amplified Fragment Length Polymorphism Analysis.- Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens.- Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex.- Multilocus Sequence Typing.- Multiple Locus Variable Number of Tandem Repeats Analysis.- Comparison of Molecular Typing Methods Applied to Clostridium difficile.- Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis.- spa Typing for Epidemiological Surveillance of Staphylococcus aureus.- Sequencing of Viral Genes.- Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterization of Foot-and-Mouth Disease Virus.- Bacterial Genome Sequencing.- DNA Microarray for Molecular Epidemiology of Salmonella.- Methods for Data Analysis.- Internet-Based Sequence-Typing Databases for Bacterial Molecular Epidemiology.
Summary
The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level.
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From the reviews:
"This book provides valuable insight into the molecular genotyping methods for differentiation and classification of microorganisms at the subspecies level … . It is intended for researchers in the field of molecular epidemiology of microorganisms. Students as well as experts in the field will find this book quite informative and useful. … This is a valuable resource providing state-of-the-art techniques applied to the investigation of transmissible diseases, enabling researchers to unravel the routes of spread of pathogens for humans, animals, and plants." (Omer Iqbal, Doody’s Review Service, September, 2009)
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From the reviews:
"This book provides valuable insight into the molecular genotyping methods for differentiation and classification of microorganisms at the subspecies level ... . It is intended for researchers in the field of molecular epidemiology of microorganisms. Students as well as experts in the field will find this book quite informative and useful. ... This is a valuable resource providing state-of-the-art techniques applied to the investigation of transmissible diseases, enabling researchers to unravel the routes of spread of pathogens for humans, animals, and plants." (Omer Iqbal, Doody's Review Service, September, 2009)
