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Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.
List of contents
Galleria mellonella as an Infection Model for Select Agents.- Drosophila as a Model for Intestinal Infections.- Zebrafish Embryos as a Model to Study Bacterial Virulence.- Studying Host-pathogen Interaction Events in Living Mice Visualized in Real Time using Biophotonic Imaging.- Intravital Two photon Imaging to Understand Bacterial Infections of the Mammalian Host.- Cre Reporter Assay for Translocation (CRAfT), A Tool for the Study of Protein Translocation into Host Cells.- Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor Tu.- Hijacking the Host Proteasome for the Temporal Degradation of Bacterial Effectors.- Life Cell Imaging of Phosphoinositide Dynamics during Legionella Infection.- Investigating Interference with Apoptosis Induction by Bacterial Proteins.- Bimolecular Fluorescence Complementation for Imaging Protein Interactions in Plant Hosts of Microbial Pathogens.- Investigating TLR Signalling Responses in Murine Dendritic Cells upon Bacterial Infection.- siRNA Screens using Drosophila Cells to Identify Host Factors Required for Infection.- Purification of Intracellular Bacteria: Isolation of Viable Brucella abortus from Host Cells.- RNA Sequencing of FACS-sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens.- Taking the Shortcut for High-throughput Shotgun Proteomic Analysis of Bacteria.- Comparative Genomic Analysis at the PATRIC, a Bioinformatic Resource Center.- A Markerless Deletion Method for Genetic Manipulation of Burkholderia cenocepacia and Other Multi Antibiotic Resistant Gram-negative Bacteria.- Gene Inactivation in Coxiella burnetii.- A Chemical Mutagenesis Approach to Identify Virulence Determinants in the Obligate Intracellular Pathogen Chlamydia trachomatis.
Summary
Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.
