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This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Immune Mediators in Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis
A Useful Guide for Analysis of Biomarkers in Cancer by Fluorochrome (Luminex) Technique.- Detection of Multi-analytes Using Luminex Multiplex Bead Immunoassay.- Flow Cytometry Analysis of IFN -induced PD-L1 Cell Surface Expression in Ovarian Cancer Cells.- Evaluating Nuclear Levels of PD-L1 in Ovarian Cancer Cells by Western Blotting.- Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells.- A Beginner s Guide to Immunohistochemistry Technique for Paraffin Sections.- Immunohistochemistry and Immunofluorescence Approaches in Cancer Research Using Paraffin-Embedded Tissue Sections.- Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.- Masson s Trichrome Staining Technique to Evaluate Tissue Fibrosis.- 3D Spheroid Model: A Methodological Approach for Advancing Cancer and Immunology Research.- Analysis of IFN -Induced Invasion of Ovarian Cancer Cells using 3D Spheroids.- Use of Biomimetic Hydrogels and 3D Cancer Models for Biochemical and Cell Biological Assays.- Detection of Ferroptosis by Liperfluo and BODIPY 581/591 C11.- Methods for Measuring TNF -induced Autophagy in Cancer Cells.- Evaluation of Metabolic Characteristics of Cancer Cells .- Assessing Intracellular Metabolism of Immune Cells in Situ in Live Zebrafish Larvae by Autofluorescence Lifetime Imaging Microscopy of NAD(P)H and FAD.- Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro.- Differentiation and Polarization of THP-1 Cells into M1 and M2 Macrophages for Cancer Research.- Analysis of PD-L1 Transcriptional Activity by Chromatin Immunoprecipitation.-
Zusammenfassung
This second edition provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches for cancer cells and tissues. Chapters beginning with the Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Immune Mediators in Cancer: Methods and Protocols, Second Edition
aims to ensure successful results in the further study of this vital field.
