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This volume discusses methods for the study of multipotent and pluripotentstem cells of the hair follicle. The stem cells described are involved in boththe growth of the hair follicle and its production of the hair shaft, as wellas the growth of the hair follicle sensory nerve. Multipotent Stem Cells ofthe Hair Follicle: Methods and Protocols also explores very unexpectedresults such as that of the hair follicle-associated-pluripotent (HAP) stemcells, which not only have the capability for regenerating the hair folliclesensory nerve, but also can differentiate ex vivo and in vivo to multiple celltypes not associated with the hair follicle-these include glial cells, motorneurons, and beating cardiac muscle cells. The potential for HAP stem cells forregenerative medicine is also discussed in detail. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Cutting-edgeand thorough, Multipotent Stem Cells of the Hair Follicle: Methods andProtocols is a valuable resource for researchers interested in thisfield.
Inhaltsverzeichnis
Introduction to Hair-Follicle-AssociatedPluripotent (HAP) Stem Cells.- Nestin-Based Reporter Transgenic Mouse Lines.- Discovery of HAP Stem Cells.- Peripheral-Nerve and Spinal-Cord Regeneration inMice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells.- Constructionof Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair FollicleNeural Crest Stem Cells.- Nestin-ExpressingHair Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term3D Gelfoam Histoculture.- Isolation and Culture of Neural Crest StemCells from Human Hair Follicles.- Isolation of Mouse Hair Follicle Bulge Stem Cellsand their Functional Analysis in a ReconstitutionAssay.- Hair Follicle Regeneration by Transplantationof a Bioengineered Hair Follicle Germ.- Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation.- Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.- Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.- Isolation and Fluorescence Activated Cell-Sorting ofMouse Keratinocytes Expressing beta-Galactosidase.- Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cordof Mice.- Long-Term Gelfoam® Histoculture ofHair-Shaft-Producing Mouse Whisker Follicles.- From Hair to Heart: Hair Follicle-Associated-Pluripotent(HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells.- Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium.- Cryopreservation of Intact Hair Follicle Maintains Pluripotencyof Nestin-Expressing (HAP) Stem Cells.
Über den Autor / die Autorin
Robert M. Hoffman, Ph.D.
Professor of Surgery, University of California, San Diego
President, AntiCancer, Inc. San Diego, CA, USA.
Zusammenfassung
This volume discusses methods for the study of multipotent and pluripotent
stem cells of the hair follicle. The stem cells described are involved in both
the growth of the hair follicle and its production of the hair shaft, as well
as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of
the Hair Follicle: Methods and Protocols also explores very unexpected
results such as that of the hair follicle-associated-pluripotent (HAP) stem
cells, which not only have the capability for regenerating the hair follicle
sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell
types not associated with the hair follicle—these include glial cells, motor
neurons, and beating cardiac muscle cells. The potential for HAP stem cells for
regenerative medicine is also discussed in detail. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Cutting-edge
and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and
Protocols is a valuable resource for researchers interested in this
field.
