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This volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field. Chapters in this book are divided into three parts: Part One focuses on visualizing myelin and studying it on a biochemical level; Part Two looks at cell culture systems used to uncover key mechanisms that regulate formation and maintenance of myelin; and Part Three discusses modeling disorders of myelin in both CNS and PNS, and therapeutic strategies for targeting these disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Myelin: Methods and Protocols is a valuable resource for physiologists, molecular biologists, anatomists, cell and developmental biologists, and physician-scientists working in the field of myelin biology and the disorders associated with it.
Inhaltsverzeichnis
Electron Microscopy for the Analysis of Peripheral Nerve Myelin.- Immunohistochemical Analysis of Myelin Structures.- Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish.- Lipidomics Profiling of Myelin.- Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry.- Proteomic Analysis of Myelin Composition.- Isolation and Purification of Primary Rodent Schwann Cells.- Isolation, Expansion, and Maturation of Oligodendrocyte Lineage Cells Obtained from Rat Neonatal Brain and Optic Nerve.- DRG Neuron/Schwann Cells Myelinating Co-Cultures.- Neuron/Oligodendrocyte Myelination Co-Culture.- Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination.- Modeling PNS and CNS Myelination Using Microfluidic Chambers.- Modeling CNS Myelination Using Micropillar Arrays.- A Central Nervous System Axonal Myelination Assay for High-Throughput Screening.- Analyzing Autophagic Flux in Nerve Cultures.- Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro.- Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOD35-55 in the Mouse.- Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin.- Myelin Protein Zero180-199 Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice.- Transection and Crush Models of Nerve Injury to Measure Repair and Remyelination in Peripheral Nerve.- In Vivo Introduction of Transgenes into Mouse Sciatic Nerve Cells Using Viral Vectors.- Intrathecal Delivery of Viral Vectors for Gene Therapy.- Efficient Gene Transfer to Myelinating Schwann Cells in the Rodent Sciatic Nerve Using In Vivo Electroporation.
Zusammenfassung
This volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field. Chapters in this book are divided into three parts: Part One focuses on visualizing myelin and studying it on a biochemical level; Part Two looks at cell culture systems used to uncover key mechanisms that regulate formation and maintenance of myelin; and Part Three discusses modeling disorders of myelin in both CNS and PNS, and therapeutic strategies for targeting these disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Myelin: Methods and Protocols is a valuable resource for physiologists, molecular biologists, anatomists, cell and developmental biologists, and physician-scientists working in the field of myelin biology and the disorders associated with it.
