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This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
Inhaltsverzeichnis
The CE-Way of Thinking: "All is Relative!".- A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.- Derivatization in Capillary Electrophoresis.- Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis-Strategies and Implementation.- Micellar Electrokinetic Chromatography of Aminoglycosides.- Microemulsion Electrokinetic Chromatography.- Non Aqueous Capillary Electrophoresis Mass Spectrometry.- Ionic Liquids in Capillary Electrophoresis.- CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.- Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry.- Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells and Rhizosphere.- Analysis of Small Ions with Capillary Electrophoresis.- Metal Ions Analysis with Capillary Zone Electrophoresis.- Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis.- Enantiomer Separations by Capillary Electrophoresis.- Capillary Electrophoresis of Mono- and Oligosaccharides.- Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.- Separation of Peptides by Capillary Electrophoresis.- Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach.- Capillary Electrophoretic Analysis of Classical Organic Pollutants.- Capillary Electrophoresis in Metabolomics.- Capillary Electrophoresis in Food and Foodomics.- Capillary Electrophoresis in Wine Science.
Zusammenfassung
This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.
